"The nitrated peptides were eluted with 50% acetonitrile/0. 2% trifluoroacetic acid, dried, resuspended in 1.5 µl of matrix suspension (4 mg α-cyano-4-hydroxycinnamic acid, 0.8 mg 3,5-dihydroxybenzoic acid in 50% acetonitrile/0. 1% trifluoroacetic acid, 0.1 mM fucose), and deposited on a stainless steel target."