"The sections were then incubated with goat antibody against GSL-1 isolectin B4 (1: 400 dilution, Vector Laboratories) for 30 min, washed with TBS and incubated with biotinylated rabbit anti-goat immunoglobulins (1: 400 dilution; Dako, Glostrup, Denmark) for 20 min in a moist chamber at room temperature."